A sodium‐stimulated ATP synthase in the acetogenic bacterium Acetobacterium woodii

FEBS Letters

Müller, Volker¹  Reidlinger, Jutta¹  Heise, Reno¹  Gottschalk, Gerhard¹ 

[1]Institut für Mikrobiologie der Universität, Grisebachstr. 8, 3400 Göttingen, Germany

关键词: Acetogenic bacterium;    Bioenergetics;    Sodium cycle;    ATP synthesis;    Na+;    Acetobacterium wooddii;    DCCD;    N;    N′-dicyclohexylcarbodiimide;    DTT;    dithiothreitol;    SF6847;    3;    5-Di-tert-butyl-4-hydroxybenzylidenemalonitrile;    ETH 2120;    N;    N;    N′;    N′-tetracyclohexyl-1;    2-phenylenedioxydiacetamide;    TCS;    3;    5;    4′;    5′-tetrachlorosalicylanilide;    THMP;    tetrahydromethanopterin;    THF;    tetrahydrofolate TRICINE;    N-tris-[hydroxymethyl] methylglycine;    ΔpH;    transmembrane chemical gradient of HCl ΔpNa;    transmembrane chemical gradient of Na+;    ΔΨ;    transmembrane electrical gradient;    ΔμfNa +;    transmembrane electrochemical gradient of Na+;   

DOI  :  10.1016/0014-5793(91)81400-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Experiments with resting cells of Acetobacterium woodii were performed to elucidate the coupling ion used by the ATP synthase. A. woodii synthesized ATP in response to an artificial ΔpH, indicating the presence of a proton-translocating ATPase. On the other hand, a ΔpNa, as well as a proton diffusion potential, could serve as a driving force for ATP synthesis with the latter strictly dependent on Na⁺. These results are indicative for the presence of a Na⁺-translocating ATP synthase in A. woodii.

【 授权许可】

Unknown