Type‐1 inhibitor of plasminogen activators

FEBS Letters

Munch, Mette¹  Andreasen, Peter A.¹  Jensen, Poul H.¹  Christian, Heegaard¹ 

[1]Department of Molecular Biology, University of Århus, 130 C.F. Moller's Allé, 8000 Århus C, Denmark

关键词: Serpin;    Plasminogen activator;    PAI-1;    Serine proteinase;   

DOI  :  10.1016/0014-5793(91)81395-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Type-1 inhibitor of plasminogen activators (PAI-I) occurs in purified preparations in a latent form that can be activated with denaturants; in vivo, latency is prevented by binding to vitronectin. We have compared latent, denaturant-activated and reactive centre-cleaved human PAI-1 with respect to thermal stability and affinity to monoclonal antibodies. By both criteria, latent and cleaved PAI-1 are very similar or indistinguishable, and clearly different from active PAI-1. Our findings suggest that the conformations of latent and reactive centre-cleaved PAI-1 are similar and resemble the so-called relaxed (R) serpin conformation, while that of active PAI-1 is different and resembles the stressed S serpin conformation.

【 授权许可】

Unknown