Relationship between DNA methylation and cell proliferation in Trypanosoma cruzi

FEBS Letters

Galanti, Norbel¹  Rojas, M.Verónica¹ 

[1] Department of Cell Biology and Genetics, School of Medicine, University of Chile, Casilla 70061, Santiago 7, Chile

关键词: DNA methylation;    5-Azacytidine;    Cell proliferation;    Trypanosoma cruzi;    5-aza-C;    5-azacytidine;    m5Cyt;    5-methylcytosine;    NDNA;    nuclear DNA;    FBS;    fetal bovine serum;    PBS;    phosphate buffered saline;    TCA;    trichloroacetic acid;    PCA;    perchloric acid;   

DOI  :  10.1016/0014-5793(91)81377-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

5-Azacytidine treatment of T. cruzi epimastigotes in culture induces active cell proliferation. This effect was detected as an increase in the cell number and [³H-methyl]thymidine incorporation into DNA. 5-Azacytidine does not alter other metabolic parameters. We have previously demonstrated that 5-azacytidine induces DNA hypomethylation in T. cruzi. Accordingly, we suggest that this chemical modification may be related to the control of T. cruzi cell division.

【 授权许可】

Unknown