A novel strategy for production of a highly expressed recombinant protein in an active form

FEBS Letters

Horgan, Roger¹  Blackwell, John R.¹ 

[1] Department of Biological Sciences, The University of Wales, Aberystwyth, SY23 3DA, UK

关键词: Recombinant protein;    Activity;    DMAPP/AMP transferase;    E. coli;   

DOI  :  10.1016/0014-5793(91)81372-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Under standard growth conditions, E. coli transformed with the high-level expression vector pMON5525 produces recombinant DMAPP/AMP transferase in inactive, insoluble complexes. We have produced large amounts of active, soluble protein by growing and inducing the cells under osmotic stress in the presence of sorbitol and glycyl betaine. This caused an increase of up to 427-fold in the active yield, and the disappearance of the protein from the pelletable fraction of cell extracts. This treatment may have wide applicability.

【 授权许可】

Unknown