2‐Deoxy‐2‐fluoro‐D‐galactose protein N‐glycosylation

FEBS Letters

Loch, Nikolaus¹  Spörndle, Irmgard¹  Geilen, Christoph C.¹  Tauber, Rudolf³  Oberdorfer, Franz²  Reutter, Werner¹  Keppler, Dietrich² 

[1] Institut für Molekularbiologie und Biochemie der Freien Universität Berlin, Arnimallee 22, D-1000 Berlin 33 Dahlem, Germany;Deutsches Krebsforschungszentrum, D-6900 Heidelberg, In: Neuenheimer Feld, Germany;Institut für Klinische Chemie und Biochimie, Klinikum Rudolf Virchow der Freien Universität Berlin, D-1000 Berlin 19, Spandauer Damm, Germany

关键词: Inhibition of N-glycosylation;    Glycoprotein;    Tunicamycin;    2-Deoxy-2-fluoro-D-galactose;    2-Deoxy-2-fluoro-D-glucose;    Hepatocyte;    dGalF;    2-deoxy-2-fluoro-D-galactose;    dGlcF;    2-deoxy-2-fluoro-D-glucose;    Dol-P;    dolichyl monophosphate;    Dol-PP;    dolichyl pyrophosphate;    Fuc;    fucose;    Gal;    galactose;    GlcNAc;    N-acetyl-glucosamine;    NeuAc;    N-acetylneuraminic acid;    PAGE;    polyacrylamide gel electrophoresis;    PBS;    phosphate buffered saline;    SDS;    sodium dodecysulfate;   

DOI  :  10.1016/0014-5793(91)81433-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

2-Deoxy-2-fluoro-D-galactose (dGalF), added to the medium of primary cultured rat hepatocytes, inhibited N-glycosylation on membrane (gp 120) and secretory glycoproteins (α ₁-macroglobulin) in a concentration dependent manner. Complete inhibition of N-glycosylation was achieved at concentrations of 1 mM and above. At identical concentrations, 2-deoxy-2-fluoro-D-glucose (dGlcF) caused only incomplete inhibition of N-glycosylation. dGalF reduced incorporation of D-[2,6-³H]mannose into lipid-linked oligosaccharides indicating interference with their assembly in the dolichol cycle.

【 授权许可】

Unknown