Fragmentation of proteins by S. aureus strain V8 protease

FEBS Letters

Sørensen, Steen Bech¹  Sørensen, Thomas Lykke¹  Breddam, Klaus¹ 

[1] Carlsberg Laboratory, Department of Chemistry, Gl. Carlsberg Vej 10, DK-2500 Copenhagen Valby, Denmark

关键词: Staphylococcus aureus;    Protease;    Serine endopeptidase;    Specificity;    Amino acid sequence analysis;    ABz;    o-aminobenzoyl (anthraniloyl);    CAPS;    3-(cyclohexylamino)propanosulphonic acid;    CHES;    2-(cyclohexylamino)ethanesulphonic acid;    TFA;    trifluoroacetic acid;   

DOI  :  10.1016/0014-5793(91)80667-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Staphylococcus aureus strain V8 protease is a serine endopeptidase which cleaves peptide bonds at the carboxyl side of Glu and Asp. Specific cleavage at Glu has previously been achieved in ammonium bicarbonate whereas in sodium phosphate cleavage at both Glu and Asp was observed. However, it is shown here that bicarbonate does not restrict the specificity to Glu-X bonds, it simply inhibits the enzyme. The degradation of a mixture of oxidized insulin and glucagon proceeds similarly in the two buffers, although faster in phosphate.

【 授权许可】

Unknown