FEBS Letters | |
Interaction of intact type VI collagen with hyaluronan | |
Tucker, Lynne1  Marcelino, Jose1  McDevitt, Cahir A.1  | |
[1] Department of Musculoskeletal Research, The Cleveland Clinic Foundation Research Institute, Cleveland, OH 44195, USA | |
关键词: Collagen; Type VI collagen; Hyaluronan; Hyaluronic acid; Cartilage; Meniscus; PEG; polyethylene glycol; PMSF; phenylmethylsulfonyl fluoride; GuHCl; glanidine hydrochloride; BSA; bovine serum albumin; | |
DOI : 10.1016/0014-5793(91)80660-U | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The capacity of non-pepsinyzed type VI collagen to oind to hyaluronan was investigated. Type VI collagen was extracted from bovine meniscal cartilage with 6 M GuHCl and purified by extraction of PEG precipitates and dissociative Sephacryl S-500 HR chromatography. Type VI collagen, detected with a monoclonal antibody, bound in 0.5 M NaCl to hyaluronan-coated micro-wells, the degree of binding being higher at 37°C than 23°C and 4°C. Incubation of type VI collagen in competitive inhibition assays with testicular hyaluronidase digests of hyaluronan in liquid phase, reduced binding of the protein to hyaluronan-coated microwells to background levels. Thus, non-pepsinyzed type VI collagen binds to hyaluronan in vitro.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020295694ZK.pdf | 391KB | download |