Interaction of intact type VI collagen with hyaluronan

FEBS Letters

Tucker, Lynne¹  Marcelino, Jose¹  McDevitt, Cahir A.¹ 

[1] Department of Musculoskeletal Research, The Cleveland Clinic Foundation Research Institute, Cleveland, OH 44195, USA

关键词: Collagen;    Type VI collagen;    Hyaluronan;    Hyaluronic acid;    Cartilage;    Meniscus;    PEG;    polyethylene glycol;    PMSF;    phenylmethylsulfonyl fluoride;    GuHCl;    glanidine hydrochloride;    BSA;    bovine serum albumin;   

DOI  :  10.1016/0014-5793(91)80660-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The capacity of non-pepsinyzed type VI collagen to oind to hyaluronan was investigated. Type VI collagen was extracted from bovine meniscal cartilage with 6 M GuHCl and purified by extraction of PEG precipitates and dissociative Sephacryl S-500 HR chromatography. Type VI collagen, detected with a monoclonal antibody, bound in 0.5 M NaCl to hyaluronan-coated micro-wells, the degree of binding being higher at 37°C than 23°C and 4°C. Incubation of type VI collagen in competitive inhibition assays with testicular hyaluronidase digests of hyaluronan in liquid phase, reduced binding of the protein to hyaluronan-coated microwells to background levels. Thus, non-pepsinyzed type VI collagen binds to hyaluronan in vitro.

【 授权许可】

Unknown