Phosphorylation of α‐crystallin B in Alexander's disease brain

FEBS Letters

Mann, Eric²  Chiesa, Raúl²  Goldman, James¹  Spector, Abraham²  McDermott, Martin J.² 

[1] Department of Pathology, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA;Biochemistry and Molecular Biology Laboratory, Department of Ophthalmology, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA

关键词: Lens protein;    Protein phosphorylation;    Alpha crystallin;    Alexander's disease;   

DOI  :  10.1016/0014-5793(91)81359-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The phosphorylation of α-crystallin B was studied in homogenates of autopsy samples of brain tissue from patients with Alexander's disease, a condition characterized by over-expression of this protein. After incubation in the presence of [γ-³²P]ATP and cAMP the homogenates were analyzed by two-dimensional electrophoresis, (isoelectric focusing followed by SDS-PAGE). Three major polypeptides having the same molecular weight as bovine lens α-crystallin B and pIs 7.1, 6.9 and 6.7 were detected in the Coomassie blue stained gels. These three polypeptides were recognized by an α-crystallin B-specific antiserum in Western blots. The polypeptides with pIs 7.1 and 6.7 co-migrated in isoelectric focusing gels with bovine lens αB and its phosphorylated form αBp, respectively. Radioautography of the two-dimensional gels demonstrated the presence of ³²P in the most acidic polypeptide. The results demonstrate the occurrence of αB phosphorylation in Alexander's disease brain tissue.

【 授权许可】

Unknown