Structure and expression of subunit A from the bovine chromaffin cell vacuolar ATPase

FEBS Letters

Howell, Michael¹  Strasser, Jane E.¹  Pan, Ying-Xian²  Dean, Gary E.¹  Xu, Jin¹ 

[1] Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267-0524, USA;Department of Physiology and Biophysics University of Cincinnati College of Medicine, Cincinnati, OH 45267-0524, USA

关键词: H+-ATPase;    Bovine adrenal medulla;    cDNA cloning: Subunit structure;   

DOI  :  10.1016/0014-5793(91)81158-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Subunit A of the vacuolar H⁺-ATPase class is thought to be responsible for the ATP hydrolysis which drives proton-pumping. We report here the cloning and sequence determination of the first mammalian cDNA encoding a bovine vacuolar ATPase subunit A from an adrenal medulla cDNA library. Northern blots of bovine adrenal medulla RNA reveal a message of approximately 3.8 kb. The predicted peptide sequence, consisting of 618 amino acids with a calculated molecular weight of 68397 daltons, is similar to the sequences of the three known subunit A proteins. β-Galactosidase-subunit A fusion proteins were immuno-decorated by an antiserum raised to the subunit A protein from corn coleoptile vacuoles.

【 授权许可】

Unknown