Mitochondrial preproteins en route from the outer membrane to the inner membrane are exposed to the intermembrane space

FEBS Letters

Pfanner, Nikolaus¹  Rassow, Joachim¹ 

[1] Institut für Physiologische Chemie, Universität München, Goethestr. 33, W-8000 München 2, Germany

关键词: Mitochondria;    Protein translocation;    Intermembrane space;    Contact site;    ΔΨ;    membrane potential;    GIP;    general insertion site (‘general insertion protein’);    F1β;    F1-ATPase subunitβ;    mt-hsp 70;    heat shock protein of 70 kDa in the mitochondrial matrix;   

DOI  :  10.1016/0014-5793(91)81157-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mitochondrial precursor proteins are known to be imported at sites of close contact between mitochondrial outer and inner membranes. We have identified translocation intermediates exposed to the intermembrane space, including the precursor of the ADP/ATP carrier accumulated at the general insertion site GIP, and the precursor of F₁-ATPase subunitβ accumulated on its import pathway at low levels of ATP. These results suggest that mitochondrial contact sites are not sealed structures, but that polypeptides pass (at least partly) through the intermembrane space on their route from the outer membrane to the inner membrane.

【 授权许可】

Unknown