Identification of 11‐cis and all‐trans‐retinal in the photoreceptive organelle of a flagellate green alga

FEBS Letters

Kreimer, Georg¹  Brohsonn, Uwe¹  Melkonian, Michael¹  Marner, Franz-Josef² 

[1] Universität zu Köln, Botanisches Institut, Lehrstuhl I, Gyrhofstr. 15, D-5000 Köln 41, Germany;Universität zu Köln, Institut für Biochemie, Zülpicher Str. 47, D-5000 Köln 41, Germany

关键词: Retinal;    Phototaxis;    Photoreceptive organelle;    Eyespot;    Green algae;   

DOI  :  10.1016/0014-5793(91)81150-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Isolation of intact photoreceptive organelles (eyespot apparatuses) involved in blue-light mediated photoresponses in a flagellate green alga (Spermatozopsis similis) allowed for the first time the identification of both 11-cis- and all-trans-retinal in a plant cell. Both isomers were identified by HPLC analysis in conjunction with UV spectra. Additionally, reconstitution of a distinct absorption band, centered around 540 nm, was achieved by addition of exogenous 9-cis-retinal to bleached, isolated eyespot apparatuses.

【 授权许可】

Unknown