Effects of 17‐hydroxywortmannin on serine/threonine‐protein kinases in human blood platelets

FEBS Letters

Kocher, Markus¹  Clemetson, Kenneth J.¹ 

[1] Theodor Kocher Institute, University of Berne, Freiestrasse 1, CH-3012 Berne, Switzerland

关键词: Platelet;    Protein kinase;    Aggregation;    Signal transduction;    Thrombin;    Fungal metabolite;    HWT;    17-hydroxywortmannin;    PVDF;    polyvinylidene difluoride;    fMLP;    N-formyl-methionylleucylphenylalanine;    PMA;    phorbol myristyl acetate;    IWT;    inactive wortmannin derivative;   

DOI  :  10.1016/0014-5793(91)81321-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein kinases are involved in signal transduction in human blood platelets. A number of renaturable protein kinases have increased in vitro activities compared to controls due to covalent modifications when intact platelets are activated by thrombin. The effect of the platelet inhibitor 17-hydroxywortmannin (HWT) on these protein kinases was investigated in intact platelets and in vitro. HWT inhibits the increase in activity of these protein kinases but it does not interact directly with their catalytic subunits. We conclude that HWT blocks a step in signal transduction which is necessary to activate these protein kinases via covalent modifications.

【 授权许可】

Unknown