Molecular mechanism of protein kinase C modulation of sodium channel α‐subunits expressed in Xenopus oocytes

FEBS Letters

Dascal, N.¹  Weigl, L.²  Lotan, I.¹  Wallner, M.²  Schreibmayer, W.² 

[1] Department of Physiology and Pharmacology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel;Institute of Medical Physics and Biophysics, Karl-Franzens University Graz, A-8010 Graz, Austria

关键词: Sodium channel modulation;    Protein kinase C (PKC);    Phorbol ester;    Xenopus laevis;    Patch clamp;    RNA expression;   

DOI  :  10.1016/0014-5793(91)81316-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mechanism of modulation of sodium channel α-subunits (Type IIA) by a protein kinase C (PKC) activator was studied on single channel level. It was found that: (i) time constants for channel activation were prolonged; (ii) inactivation remained virtually unchanged; (iii) peak sodium inward current was reduced as evidenced by calculation of average sodium currents; and (iv) time constants for current activation and decay were prolonged. (i), (iii) and (iv) were voltage dependent, being most prominent at threshold potentials. The data show that a voltage dependent action on the activation gate can account for the observed reduction of peak inward sodium current and prolongation of current decay in macroscopic experiments.

【 授权许可】

Unknown