Calcium influx through ‘L’‐type channels into rat anterior pituitary cells can be modulated in two ways by protein kinase C (PKC‐isoform selectivity of 1,2‐dioctanoyl sn‐glycerol?)

FEBS Letters

MacEwan, David J.¹  Mitchell, Rory¹ 

[1] MRC Brain Metabolism Unit, 1 George Square, Edinburgh EH8 9JZ, UK

关键词: Phorbol ester;    Diacylglycerol;    Protein kinase C isoform;    Ca2+ influx;    Rat anterior pituitary cell;    PKC;    protein kinase C;    EGTA;    ethyleneglycol-bis-(β-aminoethyl ether)N;    N;    N′;    N′-tetraacetic acid;    PDBu;    phorbol 12;    13-dibutyrate;    4α-PDD;    4α-phorbol 12;    13-didecanoate;    4β-PDD;    4β-phorbol 12;    13-didecanoate;    DOG;    1;    2-dioctanoyl sn-glycerol;    OAG;    1-olcoyl 2-acetyl sn-glycerol;   

DOI  :  10.1016/0014-5793(91)81108-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The depolarisation-induced influx of ⁴⁵Ca²⁺ into anterior pituitary tissue and GH₃ cells through ‘L’-type, nimodipine-sensitive channels was investigated. In anterior pituitary prisms, phorbol esters, activators of protein kinase C, caused an enhancement of K⁺-induced ⁴⁵Ca²⁺ influx. However, in the GH₃ anterior pituitary cell line, phorbol esters inhibited K⁺-induced ⁴⁵Ca²⁺ influx. The modulation by phorbol esters in both tissues was stereo-specific and time- and concentration-dependent. The diacylglycerol analogue, 1,2-dioctanoyl sn-glycerol was able to mimic the phorbol ester-induced enhancement of calcium influx into anterior pituitary pieces, but was ineffective in GH₃ cell. 1,2-Diactanoyl sn-glycerol may selectively activate an isoform of protein kinase C which is responsible for enhanced ‘L’-type Ca²⁺-channel activity.

【 授权许可】

Unknown