Calponin Developmental isoforms and a low molecular weight variant

FEBS Letters

Celis, J.E.²  Small, J.V.¹  Gimona, M.¹  Draeger, A.¹  Stuckert, A.¹ 

[1] Institute of Molecular Biology of the Austrian Academy of Sciences, Salzburg, Austria;Institute of Medical Biochemistry, Aarhus University, Aarhus, Denmark

关键词: Smooth muscle;    Protein isoforms;    Development;    Calponin;   

DOI  :  10.1016/0014-5793(91)81095-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two-dimensional gel analysis of basic proteins in developing human smooth muscle identifies calponin as a prominent marker of the differentiated phenotype. Adult tissue (human and mouse) typically expresses up to four calponin isoforms, three of which appear sequentially during fetal development: adult myometrial cells express the same three isoforms in primary culture in vitro and these are down-regulated, in reverse order, during the subsequent modulation of phenotype. Monospecific, polyclonal antibodies against calponin identify a lower molecular weight variant of calponin (L-calponin) that is strongly and specifically expressed in adult smooth muscles of the human urogenital tract. L-Calponin is down-regulated in benign smooth muscle derived tumors (leiomyoma) and is not expressed in primary cultures of normal uterine tissue.

【 授权许可】

Unknown