Tetanus toxin receptor Specific cross‐linking of tetanus toxin to a protein of NGF‐differentiated PC 12 cells

FEBS Letters

Montecucco, Cesare¹  Rossetto, Ornella¹  Ferrari, Giovanna²  Schiavo, Giampietro¹ 

[1] Centro CNR Biomembrane e Istituto di Patologia Generale, Via Trieste, 75 Padova, Italy;Laboratori di Ricerca Fidia, Via Ponte della Fabbrica, Abano Terme, Italy

关键词: Tetanus toxin;    Protein receptor;    Pheochromocytoma cell;    Nerve growth factor;    Cross-linker;    PC 12 cells;    rat pheochromocytoma cell line;    NGF;    nerve growth factor;    DSS;    disuccinimidyl suberate;    DSP;    dithiobis-(succinimidylpropionate);    BSOCOES;    Bis-2- (succinimidooxycarbonyloxy)ethyl sulfone;    HBS;    Hanks Balanced Solution supplemented with 1.1 g/l glucose;    PMSF;    phenylmethyisulfonyl fluoride;    TPCK;    n-tosyl-L-phenylalanine chloromethyl ketone;   

DOI  :  10.1016/0014-5793(91)81266-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A subclone of rat pheochromocytoma cells expresses high affinity receptors for tetanus toxin on differentiation with NGF [Walton, K.M., Sandberg, K., Rogers, T.B. and Schnaar, R.L. (1988) J. Biol. Chem. 263, 2055–2063]. In the presence of protein cross-linking agents, [¹²⁵I]tetanus toxin, bound to these cells at 0°C, forms a cross-linked product with apparent molecular weight of 120 kDa. The formation of [¹²⁵I]tetanus toxin conjugate involves the heavy chain of the toxin, is prevented by cold toxin and it is largely reduced by pretreating cells with proteases, The cross-linked product is formed only upon incubation of the toxin with NGF-differentiated cells. These results suggest that a protein with apparent molecular weight of 20 kDa is involved in the neurospecific binding of tetanus toxin.

【 授权许可】

Unknown