FEBS Letters | |
Tetanus toxin receptor Specific cross‐linking of tetanus toxin to a protein of NGF‐differentiated PC 12 cells | |
Montecucco, Cesare1  Rossetto, Ornella1  Ferrari, Giovanna2  Schiavo, Giampietro1  | |
[1] Centro CNR Biomembrane e Istituto di Patologia Generale, Via Trieste, 75 Padova, Italy;Laboratori di Ricerca Fidia, Via Ponte della Fabbrica, Abano Terme, Italy | |
关键词: Tetanus toxin; Protein receptor; Pheochromocytoma cell; Nerve growth factor; Cross-linker; PC 12 cells; rat pheochromocytoma cell line; NGF; nerve growth factor; DSS; disuccinimidyl suberate; DSP; dithiobis-(succinimidylpropionate); BSOCOES; Bis-2- (succinimidooxycarbonyloxy)ethyl sulfone; HBS; Hanks Balanced Solution supplemented with 1.1 g/l glucose; PMSF; phenylmethyisulfonyl fluoride; TPCK; n-tosyl-L-phenylalanine chloromethyl ketone; | |
DOI : 10.1016/0014-5793(91)81266-B | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A subclone of rat pheochromocytoma cells expresses high affinity receptors for tetanus toxin on differentiation with NGF [Walton, K.M., Sandberg, K., Rogers, T.B. and Schnaar, R.L. (1988) J. Biol. Chem. 263, 2055–2063]. In the presence of protein cross-linking agents, [125I]tetanus toxin, bound to these cells at 0°C, forms a cross-linked product with apparent molecular weight of 120 kDa. The formation of [125I]tetanus toxin conjugate involves the heavy chain of the toxin, is prevented by cold toxin and it is largely reduced by pretreating cells with proteases, The cross-linked product is formed only upon incubation of the toxin with NGF-differentiated cells. These results suggest that a protein with apparent molecular weight of 20 kDa is involved in the neurospecific binding of tetanus toxin.
【 授权许可】
Unknown
【 预 览 】
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