Cloning, expression and chromosomal localization of a new putative receptor‐like protein tyrosine phosphatase

FEBS Letters

Suijkerbuijk, Ron¹  Beijersbergen, Roderick L.²  Moolenaar, Wouter H.²  Hateboer, Guus²  Gebbink, Martijn F.B.G.²  van Kessell, Ad Geurts¹  van Etten, Ingrid² 

[1] Department of Human Genetics, University Hospital, Postbus 91011 6500 HB Nijmegen, The Netherlands;Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands

关键词: Receptor-like tyrosine phosphatase;    PTP;    protein tyrosine phosphatase;    RPTP;    receptor-like protein tyrosine phosphatase;    PTK;    protein tyrosine kinase;    FN;    fibronectin;    PCR;    polymerase chain reaction;    LAR;    leukocyte common antigen-related molecule;   

DOI  :  10.1016/0014-5793(91)81241-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated a mouse cDNA of 5.7 kb, encoding a new member of the family of receptor-like protein tyrosine phosphatases, termed mRPTPμ. The cDNA predicts a protein of 1432 amino acids (not including signal peptide) with a calculated M _r of 161 636. In addition, we have cloned the human homologue, hRPTPμ, which shows 98.7% amino acid identity to mRPTPμ. The predicted mRPTPμ protein consists of a 722 amino acid extracellular region, containing 13 potential N-glycosylation sites, a single transmembrane domain and a 688 amino acid intracellular part containing 2 tandem repeats homologous to the catalytic domains of other tyrosine phosphatases. The N-terminal extracellular part contains a region of about 170 amino acids with no sequence similarities to known proteins, followed by one Ig-like domain and 4 fibronectin type III-like domains. The intracellular part is unique in that the region between the transmembrane domain and the first catalytic domain is about twice as large as in other receptor-like protein tyrosine phosphatases. RNA blot analysis reveals a single transcript, that is most abundant in lung and present in much lower amounts in brain and heart. Transfection of the mRPTPμ cDNA into COS cells results in the synthesis of a protein with an apparent M_r of 195 000, as detected in immunoblots using an antipeptide antibody. The human RPTPμ gene is localized on chromosome 18pter-q11, a region with frequent abnormalities implicated in human cancer.

【 授权许可】

Unknown