Immunological demonstrations of εPKC Murine tissue distribution, ontogeny, cellular localization and translocation

FEBS Letters

Marks, Friedrich¹  Gschwendt, Michael¹  Rincke, Gabriele¹  Leibersperger, Hanno¹ 

[1] Institute of Biochemistry, German Cancer Research Center, Im Neuenheimer Feld 280, D-6900 Heidelberg, Germany

关键词: ε-Protein kinase C;    Antiserum;    Mouse brain;    Ontogeny;    Translocation;    PKC;    protein kinase C;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    ELISA;    enzyme-linked immunosorbent assay;   

DOI  :  10.1016/0014-5793(91)81239-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An antiserum raised against an εPKC-specific peptide recognizes εPKC with an apparent molecular weight of 97 kDa in cytosol of mouse brain. No cross-reaction with α,β,γPKC or the δPKC-like p76-kinase is observed. εPKC is mainly present in brain. Just traces of this PKC isoenzyme can be detected in some other murine tissues. Ontogenetic studies indicate that the amount of εPKC in murine brain increases constantly and reaches a maximal level at day 7 after birth. Upon TPA activation εPKC is translocated from the cytosol to the particulate fraction in a brain homogenate.

【 授权许可】

Unknown