Cloning of the D‐lactate dehydrogenase gene from Lactobacillus delbrueckii subsp. bulgaricus by complementation in Escherichia coli

FEBS Letters

Ferain, Thierry¹  Garmyn, Dominique¹  Bernard, Nathalie¹  Hols, Pascal¹  Delcour, Jean¹ 

[1] Laboratoire de Génétique Moléculaire, Unité de Génétique, Université Catholique de Louvain, Croix du Sud 5 (bte 6), B-1348 Louvain-la Neuve, Belgium

关键词: D-Lactate dehydrogenase;    Insertion sequence IS2;    Lactobacillus delbrueckii subsp. bulgaricus;    D-Hydroxyisocaproate dehydrogenase;    L-LDH;    L-lactate dehydrogenase (EC 1.1.1.27);    D-LDH;    D-lactate dehydrogenase (EC 1.1.1.28);    D-HicDH;    D-hydroxyisocaproate dehydrogenase;   

DOI  :  10.1016/0014-5793(91)81226-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A strain of Escherichia coli (FMJ144) deficient for pyruvate formate lyase and lactate dehydrogenase (LDH) was complemented with a genomic DNA library from Lactobacillus delbrueckii subsp. bulgaricus. One positive clone showed LDH activity and production of D(−)lactate was demonstrated. The nucleotide sequence of the D-LDH gene (ldhA) revealed the spontaneous insertion of an E. coli insertion sequence IS2 upstream of the gene coding region. The open reading frame encoded a 333-amino acid protein, showing no similarity with known L-LDH sequences but closely related to L. casei D-hydroxyisocaproate dehydrogenase (D-HicDH).

【 授权许可】

Unknown