Distribution of glutathione peroxidases glutathione reductase in rat brain mitochondria

FEBS Letters

Ernster, Lars¹  Sandri, Gabriella²  Panfili, Enrico² 

[1] Department of Biochemistry, Arrhenius Laboratories, University of Stockholm, Sweden;Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Trieste, Italy

关键词: Glutathione reductase;    Glutathione peroxidase;    Phospholipid hydroperoxide glutathione peroxidase;    Mitochondria;    Contact site;    Rat brain;   

DOI  :  10.1016/0014-5793(91)81219-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The distribution of glutathione reductase (GR), glutathione peroxidase (GPx) and phospholipid hydroperoxide glutathione peroxidase (PHGPx) in isolated rat brain mitochondria was investigated. using a fractionation procedure for the separation of inner and outer membranes, contact sites between the two membranes and a soluble fraction mainly originating from the mitochondrial matrix. The data indicate that GR and GPx are concentrated in the soluble fraction, with a minor portion of the two enzymes being associated with the contact sites. PHGPx is localized largely in the inner membrane. The possible functional significance of these findings is discussed.

【 授权许可】

Unknown