Characterization of full‐length cDNAs and the gene coding for the human GM2 activator protein

FEBS Letters

Schröder, Maria²  Sandhoff, Konrad²  Schnabel, Doris²  Suzuki, Kunihiko¹  Tanaka, Akemi¹  Nakano, Takeshi¹  Klima, Horst² 

[1] Brain and Development Research Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599-7250, USA;Institut für Organische Chemie und Biochemie, Gerhardt-Domagk-Straße 1, 5300 Bonn 1, Germany

关键词: GM2-activator protein;    Genomic structure;    Glycolipid-binding protein;    β-hexosaminidase;    β-N-acetyl-D-hexosaminidase (EC 3.2.1.52);   

DOI  :  10.1016/0014-5793(91)81084-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Full-length cDNAs coding for the human GM2-activator protein has been isolated and characterized, and its genomic structure studied in two overlapping clones in λ-EMBL-4 isolated from a human brain genomic library. Two different cDNAs were found that were identical to the 5′-terminus to nt 1311 (counted from the A of the initiation codon, ATG) including the entire protein coding sequence. However, they were entirely dissimilar in the 3′-non-coding sequences. The genomic clones covered 94% of the full-length cDNA sequence. Three introns were found. The last exon spans contiguously the carboxyl terminus of the protein and the entire 3′-untranslated region of one of the two cDNAs with different 3′-ends. The origin of the 3′-portion of the other cDNA clone is not clear at this time.

【 授权许可】

Unknown