Cloning, pharmacological characteristics and expression pattern of the rat GABAA receptor α4 subunit

FEBS Letters

Herb, Anne¹  Keinänen, Kari¹  Seeburg, Peter H.¹  Wieland, Heike¹  Wisden, William¹  Lüddens, Hartmut¹ 

[1] Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

关键词: GABAA;    Benzodiazepine receptor;    In situ hybridization;    Thalamus;    α Subunit heterogeneity;   

DOI  :  10.1016/0014-5793(91)81076-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA of rat brain encoding the GABA_A receptor α₄ subunit has been cloned. Recombinant receptors composed of α₄, β₂ and γ₂ subunits bind with high affinity the GABA agonist [³H]muscimol and the benzodiazepine ‘alcohol antagonist’ [³H]Ro 15-4513, but fail to bind benzodiazepine agonists. The α₄ subunit is expressed mainly in the thalamus, as assessed by in situ hybridization histochemistry, and may participate in a major population of thalamic GABA_A receptors. The α₄ mRNA is found at lower levels in cortex and caudate putamen, and is rare in cerebellum.

【 授权许可】

Unknown