Detection of Na+/H+ exchanger mRNA in human neutrophils and lymphocytes using the polymerase chain reaction

FEBS Letters

Quednau, Beate²  Weiler-Güttler, Hartmut¹  Rosskopf, Dieter²  Siffert, Winfried² 

[1] Institut für Biochemie der Technischen Hochschule Darmstadt, D-6100 Darmstadt, Germany;Max-Planck-Institut für Biophysik, Kennedyalle 70, D-6000 Frankfurt/Main 70, Germany

关键词: Na+/H+ exchange;    Neutrophil;    Lymphocyte;    Polymerase chain reaction;    Hypertension;   

DOI  :  10.1016/0014-5793(91)81074-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using the reverse polymerase chain reaction (RT-PCR), we have examined the expression of Na⁺/H⁺ exchanger mRNA in human buffy coat preparations, lymphocytes and neutrophils. Total RNA from all cell types was reverse transcribed specifically and then amplified by PCR. The identity of the PCR products was confirmed by restriction enzyme analysis and hybridization with a specific oligonucleotide probe. The detection or low abundance Na⁺/H⁺ antiporter specific transcripts by RT-PCR in different human blood cells ex vivo should facilitate future studies on regulatory and pathophysiological aspects of Na⁺/H⁺ exchanger mRNA expression in human cells and tissue samples.

【 授权许可】

Unknown