Characterization of a tissue‐type plasminogen activator from porcine urine

FEBS Letters

Will, Horst¹  Zacharias, Ute¹ 

[1] Department of Enzymology, Central Institute of Molecular Biology, R. Rössle Str. 10, Berlin O-1115, Germany

关键词: Tissue-type plasminogen activator;    Urokinase-type plasminogen activator;    Monoclonal antibody;    Porcine urine;    t-PA;    tissue-type plasminogen activator;    u-PA;    urokinase-type plasminogen activator;    EIA;    enzyme-immunoassay;    mAb;    monoclonal antibody;   

DOI  :  10.1016/0014-5793(91)81058-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Porcine urine, unlike human urine, does not contain detectable amounts of urokinase-type plasminogen activator (u-PA). The plasminogen activator present in porcine urine is of tissue-type (t-PA) as identified by the following criteria. (1) Porcine urine PA exhibits an M _r of 65 000 similar to the M _r of human t-PA (64-70 000) but distinct from the M _r of human u-PA (55 000). (2) Antibodies against human t-PA bind and inhibit crude and purified porcine urine PA, while human u-PA-specific antibodies do not react with porcine urine PA. (3) Plasminogen activation by porcine urine PA is markedly stimulated in the presence of fibrinogen fragments. (4) Porcine urine PA activity is not affected by concentration of amiloride substantially suppressing human u-PA activity.

【 授权许可】

Unknown