Characterization of cDNA encoding the mouse hepatic triglyceride lipase and expression by in vitro translation

FEBS Letters

Will, Hans¹  Chang, Shau-Feng¹  Netter, Hans Jürgen¹ 

[1] Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, W-8033 Martinsried, Germany

关键词: Mouse hepatic triglyceride lipase;    cDNA cloning;    Amino acid composition;    In vitro translation;    h(m;    r)HL;    human (mouse rat) hepatic triglyceride lipase;   

DOI  :  10.1016/0014-5793(91)80910-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA coding for the mouse hepatic triglyceride lipase has been isolated from a mouse liver cDNA library. The nucleotide sequence of the cDNA shows an open reading frame encoding a polypeptide of 510 amino acids that is 91.5% and 86% homologous to rat and human hepatic lipase, respectively. The most drastic protein sequence divergence is found at the carboxyterminal end which was speculated to harbour one heparin-binding site. By in vitro translation of cRNA in the presence of pancreatic membranes the hepatic lipase was shown to be glycosylated and to have an electrophoretic mobility of 53 kDa.

【 授权许可】

Unknown