Carbachol induces secretion in a mast cell line (RBL‐2H3) transfected with the ml muscarinic receptor gene

FEBS Letters

Beaven, Michael A.¹  Jones, S.V.Penelope²  Choi, Oksoon H.¹ 

[1]Laboratory of Chemical Pharmacology, NHLBI, Bethesda, MD 20892, USA

[2]Laboratory of Biophysics and Molecular Biology, NINDS, Medical Alumni Building, University of Vermont, Burlington, VT 05405, USA

关键词: Cloned ml muscarinic receptor;    Expression (RBL-2H3 cell);    Intracellular signal;    Exocytosis;    [Ca2+]i;    concentration of free cytosolic Ca2+;    IgE;    immunoglobulin E;    EGTA;    ethylene glycol-bis-(β aminoethylether) N;    N;    N′N′-tetraacetic acid;    DNP-BSA;    dinitrophenylated bovine serum albumin;   

DOI  :  10.1016/0014-5793(91)80905-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The possibility that the ml muscarinic receptor subtype can induce release of intracellular granules and transmitters was studied by transfecting a cultured mast cell line, RBL-2H3 cells, with the ml receptor gene. Comparisons were made between carbachol- and antigen-induced activation of various secretory responses. Like antigen, carbachol stimulated inositol phospholipid hydrolysis and release of arachidonic acid with concomitant dose-dependent secretion of granular contents. Carbachol also stimulated a biphasic increase in intracellular calcium, as measured by single cell fura-2 measurements. Although the kinetics of the carbachol-induced rise in intracellular calcium differed from that induced by antigen, they both utilized the same intracellular pool of calcium, and the second phase of the rise in intracellular calcium was dependent on extracellular calcium in both cases. Thus, the ml muscarinic receptor activates release of granules by a mechanism ostensibly similar to that of antigen.

【 授权许可】

Unknown