6‐Hydroxymellein synthetase as a multifunctional enzyme complex in elicitor‐treated carrot root extract

FEBS Letters

Itoh, Masataka¹  Nishi, Arasuke¹  Kurosaki, Fumiya¹  Yamada, Mitsuhiro² 

[1] Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Sugitani, Toyama 930-01, Japan;Department of Bioscience and Technology, Hokkaido Tokai University, Minamisawa, Minami-Ku, Sapporo 005, Japan

关键词: 6-Hydroxymellein;    Multifunctional enzyme complex;    Phytoalexin;    Polyketide;    Daucus carota L;   

DOI  :  10.1016/0014-5793(91)81038-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synthetic activity of a polyketide compound 6-hydroxymellein was induced in elicitor-treated carrot root tissues. The activity was significantly inhibited by an antiserum raised against the acyl carrier protein (ACP) of fatty acid synthetase, suggesting that the enzyme(s) for 6-hydroxymellein synthesis require(s) a functional unit similar to ACP. However, the synthetic activity was not stimulated by the addition of ACP purified from Escherichia coli and was not lost even after fractionation by gel-filtration chromatography. The active fraction obtained by gel-filtration (136 kDa) was subjected to immunoblot analysis, and a 128 kDa polypeptide in the fraction was found to cross-react with anti-ACP serum. These observations suggest that the biosynthesis of 6-hydroxymellein in carrot cells is catalyzed by an enzyme consisting of a single peptide chain.

【 授权许可】

Unknown