Putative papain‐related thiol proteases of positive‐strand RNA viruses Identification of rubi‐ and aphthovirus proteases and delineation of a novel conserved domain associated with proteases of rubi‐, α‐ and coronaviruses

FEBS Letters

Gorbalenya, Alexander E.¹  Koonin, Eugene V.³  Lai, Michael M.-C.² 

[1] Institute of Poliomyelitis and Viral Encephalitides, USSR Academy of Medical Sciences, 142782 Moscow Region, USSR;Howard Hughes Medical Institute and Department of Microbiology, University of Southern California School of Medicine, Los Angeles, CA 90033, USA;Institute of Microbiology, USSR Academy of Sciences, 117811 Moscow, USSR

关键词: Papain-like protease;    RNA virus;    Polyprotein processing;    Sequence motif;    Catalytic center;    SPP;    Streptococcus pyogenes peptidase A;    CPI and CP2;    Dictyostelium discoideum cysteine proteinase 1 and 2;    actin;    Actinidia chinensis actinidin;    papain and omega;    Carica papaya proteinase I and III;    respectively;    aleur;    Hordeum vulgare aleurain;    Cat.H and L;    Rattus norvegicus cathepsins H and L;    Cat.B;    Homo sapiens cathepsin B;    SH-EP;    Vigna mungo cysteine endopeptidase;    bromel;    Ananas comosus bromelain;    derpt;    Dermatophagoides pteronyssinus major mite fecal allergen;    calp;    Mus musculus calpain;    SFV;    Semliki forest virus;    SNBV;    Sindbis virus;    VEEV;    Venezuelan equine encephalomyelitis virus;    ONNV;    O'Nyong-Nyong virus;    RRV;    Ross River virus;    MidV;    Middelburg virus (alphaviruses);    PVY;    potato virus Y;    PPV;    plum pox virus;    TEV;    tobacco etch virus;    TVMV;    tobacco vein mottling virus (potyviruses);    BaYMV;    barley yellow mosaic virus (bymovirus);    MHV;    murine hepatitis virus;    IBV;    avian bronchitis virus (coronaviruses);    FMDV A10;    foot-and-mouth-disease virus A10 strain (aphthovirus);    RuV;    rubella virus (rubivirus);    HC;    helper component;    M-pro;    ‘main’ protease;    L-pro;    ‘leader’;    or accessory protease (see text);    SPL;    ‘Streptococcus-like’ protease;    Cl;    cylindrical inclusion (potyvirus protein);   

DOI  :  10.1016/0014-5793(91)81034-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A computer-assisted comparative analysis of the amino acid sequences of (putative) thiol proteases encoded by the genomes of several diverse groups or positive-stranded RNA viruses and distantly related to the family of cellular papain-like proteases is presented. A high level of similarity was detected between the leader protease of foot-and-mouth-disease virus and the protease of murine hepatitis coronavirus which cleaves the N-terminal p28 protein from the polyprotein. Statistically significant alignment of a portion of the rubella virus polyprotein with cellular papain-like proteases was obtained, leading to tentative identification of the papain-like protease as the enzyme mediating processing of the non-structural proteins of this virus. Specific grouping between the sequences of the proteases of α-viruses, and poty- and bymoviruses was revealed. It was noted that papain-like proteases of positive-stranded RNA viruses are much more variable both in their sequences and in genomic locations than chymotrypsin-related proteases found in the same virus class. A novel conserved domain of unknown function has also been identified which flanks the papain-like proteases of α-, rubi- and coronaviruses.

【 授权许可】

Unknown