Molecular cloning, nucleotide sequence and expression of the tufB gene encoding elongation factor Tu from Thermus thermophilus HB8

FEBS Letters

Hakoshima, Toshio¹  Tomita, Ken-ichi¹  Kushiro, Akira¹  Satoh, Motohide¹  Tanaka, Toshikazu¹ 

[1] Faculty of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565, Japan

关键词: Elongation factor Tu;    tufB gene;    Thermus thermophilus HB8;    GTP-binding protein;    Codon usage;    Expression;   

DOI  :  10.1016/0014-5793(91)81011-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The tufB gene encoding elongation factor Tu (EF-Tu) of Thermus thermophilus HB8 was cloned and expressed. Compared with the known tufA gene of T. thermophilus, nucleotide differences were found at 10 positions out of 1221 nucleotides, and amino acid substitutions were found at 4 positions out of 406 amino acids. The tufB product was 70.9% homologous to the corresponding sequence of the tufB product of E. coli. The G+C content of the third base of the codon in the tufB gene was 84.8% and G was especially preferred in this position.

【 授权许可】

Unknown