Adipogenesis inhibitory factor A novel inhibitory regulator of adipose conversion in bone marrow

FEBS Letters

Mita-Honjo, Katsuko¹  Takiguchi, Yo¹  Sakakibara, Sachiko¹  Ohsumi, Jun¹  Kawashima, Ichiro¹  Ishikawa-Ohsumi, Hiroko¹  Miyadai, Kenji¹ 

[1] Bioscience Research Laboratories, Sankyo Co. Ltd., 2-58, Hiromachi 1-chome, Shinagawa-ku, Tokyo 140, Japan

关键词: Adipogenesis inhibitory factor;    Interleukin-11;    Adipocyte differentiation;    Lipoprotein lipase;    Bone marrow stromal cell;   

DOI  :  10.1016/0014-5793(91)80991-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recombinant adipogenesis inhibitory factor (AGIF) was purified to homogeneity from the conditioned medium of COS-1 cells transfected with human AGIF cDNA. The amino-terminal sequence analysis of the mature AGIF revealed that AGIF was produced as a precursor consisting of 199 amino acids and processed into a mature form of 178 amino acids by a cleavage between Ala(−1) and Pro(+1). The purified AGIF inhibited the process of adipogenesis in mouse 3T3-L1 preadipocytes, indicating that AGIF directly acts on the cells. AGIF acted as an adipogenic antagonist not only on the extramedullary cell line 3T3-L1 but also on the mouse bone marrow stroma-derived cell line H-I/A, suggesting that this cytokine may regulate adipogenesis in bone marrow.

【 授权许可】

Unknown