Solubilization of rat kidney lysosomes in reversed micelles of aerosol OT in octane

FEBS Letters

Wiederschain, German Ya.¹  Buneeva, Olga A.¹  Pshezhetsky, Alexey V.² 

[1] Institute of Biological and Medical Chemistry, USSR Academy of Medical Sciences, Moscow, USSR;Belozersky Laboratory, Moscow State University, Moscow, USSR

关键词: Lysosomal glycosidase;    N-Acetyl-β-D-hexosaminidase;    Micellar enzymology: Reversed micelle;    AOT;    Aerosol OT (sodium bis(2-ethylhexyl)sulfo-succinate;    BSA;    bovine serum albumin;    EDTA;    ethylene diaminc tetraacetic acid;    4-MufGal;    4-methylumbelliferyl-β-D-galaclopyrano-side;    4-MufClcNAc;    4-methylumbelliferyl-N-acetyl-β-D-glucosaminidide;    4-MufFuc;    4-methylumbelliferyl-α-L-fucoside;    pNPGlcNAc;    p-nitrophenyl-N-acetyl-β-D-glucosaminidide;    Triton X-100;    (4-octyl)-phenol poly(9–10)ethylene glycol;    Tris;    tris-(hydroxymethyl)-aminomethan;   

DOI  :  10.1016/0014-5793(91)80055-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Intact lysosomes from rat kidneys were solubilized in the ternary system: surfactant (Aerosol OT)-buffer-organic solvent. According to data of laser light-scattering analysis and kinetic experiments with the lysosomal marker enzyme, N-acetyl-β-D-hexosaminidase (EC 3.2.1.30), the solubilization of lysosomes in this system resulted in the destruction of the lysosomes and the entrapping of their components in reversed micelles.

【 授权许可】

Unknown