Porcine leukocyte 5‐ and 12‐lipoxygenases are iron enzymes

FEBS Letters

Ullrich, Volker²  Yamamoto, Shozo¹  Kroneck, Peter M.H.²  Yoshimoto, Tanihiro¹  Ueda, Natsuo¹  Matsuda, Satoshi¹  Cucurou, Christophe²  Suzuki, Hiroshi¹ 

[1] Department of Biochemistry, Tokushima University, School of Medicine, Tokushima 770, Japan;Fakultät für Biologie, Universität Konstanz, Postfach 5560, 7750 Konstanz, Germany

关键词: Electron paramagnetic resonance;    Iron enzyme: Lipoxygenase;    Porcine leukocytes;    EPR;    electron paramagnetic resonance;    EXAFS;    extended X-ray absorption fine structure;    12-HPETE;    12-hydroperoxy-eicosatetraenoic acid;    9-HPOD;    9-hydroperoxyoctadecadienoic acid;    NDGA;    nordihydroguaiaretic acid;    DETAPAC;    diethylenetriamine-pentaacetic acid;    SP-HPLC;    straight phase high performance liquid chromatography;   

DOI  :  10.1016/0014-5793(91)80026-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

5- and 12-lipoxygenases isolated from porcine leukocytes were investigated by electron paramagnetic resonance at X-band and atomic absorption spectroscopy. For comparison potato 5-lipoxygenase was studied under identical experimental conditions. All three lipoxygenases contained between 0.7 and 0.9 Fe atoms/enzyme molecule. As isolated, both mammalian enzymes exhibited a characteristic EPR signal at low magnetic field with a maximum at g =5.20 indicative of a high-spin ferric iron center. The signal was not affected by the oxidants 12-hydroperoxyeicosatetraenoic acid or arachidonic acid, nor was it affected by the reductant nordihydroguaiaretic acid. In the case of the potato enzyme an intense EPR signal with resonances at g =7.50, 6.39 and 5.84 was only observed after addition of an oxidant, such as 9-hydroperoxyoctadecadienoic acid.

【 授权许可】

Unknown