Degradation of transcription factors, c‐Jun and c‐Fos, by calpain

FEBS Letters

Hirai, Syu-ichi²  Yaniv, Moshe¹  Kawasaki, Hiroshi²  Suzuki, Koichi² 

[1] Department of Biotechnology Pasteur Institute, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France;Department of Molecular Biology, The Tokyo Metropolitan Institute of Medical Science 3-18-22, Honkomagome, Bunkyo-ku, Tokyo 113, Japan

关键词: c-Jun;    c-Fos;    Calpain;    Calpastatin;    PEST;    Calcium;   

DOI  :  10.1016/0014-5793(91)80015-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

c-Jun protein, and AP1/PEA1 transcription factor component, is a typical short-lived protein, and like other short-lived proteins such as c-Fos, contains PEST regions. Calcium-dependent neutral protease (calpain), a candidate for the degradation of PEST-containing proteins, digests c-Jun and c-Fos efficiently in vitro. This is the first demonstration that transcription factors are substrates for calpain. The C-terminal portion of c-Jun is relatively resistant to calpain such that an 18kDa fragment, which includes the DNA binding domain, accumulates under moderate digestion conditions. The activity of c-Jun in cultured cells can be modified by changing the level of calpastatin, an endogenous calpain inhibitor, indicating that c-Jun is also a substrate for calpain in vivo.

【 授权许可】

Unknown