Cloning and expression of human endothelin‐1 receptor cDNA

FEBS Letters

Hosoda, Kiminori¹  Mukoyama, Masashi¹  Suga, Shin-ichi¹  Imura, Hiroo¹  Ogawa, Yoshihiro¹  Shirakami, Gotaro¹  Saito, Yoshihiko¹  Nakao, Kazuwa¹  Nakanishi, Shigetada² 

[1] Second Division, Department of Medicine Kyoto University School of Medicine, Kyoto 606, Japan;Institute for Immunology, Kyoto University School of Medicine, Kyoto 606, Japan

关键词: Endothelin (ET);    Receptor;    cDNA cloning: G-protein;    Xenopus oocyte;    Northern blot hybridization analysis;    ET;    endothelin;    IC50;    50% inhibitory concentration;   

DOI  :  10.1016/0014-5793(91)80007-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We isolated a human endothelin-1 (ET-1) receptor cDNA from a human placenta cDNA library. The cDNA encodes a 427-amino acid protein with seven putative transmembrane domains. The rank order of the binding to the receptor expressed in COS-7 cells was: ET-1 ⩾ ET-2 ⪢ ET-3. The receptor expressed in Xenopus oocytes showed a potent electrophysiological response to 1 × 10⁻⁷ M ET-1 under voltage clamp at −60 mV, while a much weaker response was produced by 1 × 10⁻⁷ M ET-3. Northern blot analysis with RNA from human tissues revealed a single band with a size of 4.3 kb in a wide variety of human tissues, especially highly in the blood vessel.

【 授权许可】

Unknown