Cloning and sequencing the urocanase gene (hutU) from Pseudomonas putida

FEBS Letters

Fessenmaier, Martin¹  Frank, Rainer²  Retey, János¹  Schubert, Carsten¹ 

[1] Chair of Biochemistry, Institute of Organic Chemistry, University of Karlsruhe, D-750O Karlsruhe, Germany;Center for Molecular Biology, University of Heidelberg, D-6900 Heidelberg, Germany

关键词: Urocanase;    hutU Gene;    PCR;    Pseudomonas putida;   

DOI  :  10.1016/0014-5793(91)80938-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A clone harbouring the entire urocanase gene (hutU) was obtained from a genomic library of Pseudomonas putida using oligonucleotide probes synthesised on the basis of known flanking sequences. One subunit of urocanase consists of 556 amino acids and has a molecular mass of 60 771 Da.

【 授权许可】

Unknown