Photoreversible antigen—antibody reactions

FEBS Letters

Nakanishi, Mamoru²  Sisido, Masahiko¹  Harada, Masataka¹  Hirose, Junzo² 

[1]Research Laboratory of Resources Utilization, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 227, Japan

[2]Faculty of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467, Japan

关键词: Monoclonal antibody;    Photochromic hapten;    Azobenzene;    Photocontrol;   

DOI  :  10.1016/0014-5793(91)80928-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A monoclonal antibody (Z1H01) for an oligopeptide carrying an azobenzene group, was prepared under conditions where the azobenzene group is in the trans form. The antibody bound the hapten peptide effectively when the hapten peptide is in the trans form (K=5 × 10⁷ M⁻¹), but the antibody released the hapten under irradiation with UV light where the hapten is in the cis form. The antibody bound the hapten again, when the hapten reverted to the trans form after irradiation with visible light.

【 授权许可】

Unknown