Heterologous in vitro transcription from two archaebacterial promoters

FEBS Letters

Hüdepohl, Uwe¹  Horne, Mary¹  Gropp, Felix²  Zillig, Wolfram¹ 

[1] Max-Planck Institut für Biochemie, 8033 Martinsried b. München, Germany;University of California, School for Medicine, Department of Biochemistry, HSE-1556 San Francisco, USA

关键词: Transcription initiation;    Cell-free extract;    Promoter;    Evolution;    Phage ΦH;    Gas vacuole gene;   

DOI  :  10.1016/0014-5793(91)80811-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cell-free extract of Sulfolobus shibatae is able to specifically initiate transcription in vitro at the promoter of the plasmid-encoded gene for the major gas vesicle protein of Halobacterium halobium and at the promoter for the transcript T4 of the temperate H. halobium phage ΦH. The corresponding promoter from the virulent phage mutant ΦHL1 yields enhanced transcription in the heterologous system, in agreement with strongly increased in vivo expression.

【 授权许可】

Unknown