The specific production of the third component of complement by osteoblastic cells treated with 1α,25‐dihydroxyvitamin D3

FEBS Letters

Suda, Tatsuo¹  Hong, Mei Hua¹  Udagawa, Nobuyuki¹  Ishimi, Yoshiko¹  Jin, Cheng He¹  Shinki, Toshimasa¹  Abe, Etsuko¹  Sato, Toshiyuki¹ 

[1] Department of Biochemistry, School of Dentistry, Showa University, Tokyo 142 Japan

关键词: Complement C3;    1α;    25-Dihydroxyvitamin D3;    Osteoblastic cell;   

DOI  :  10.1016/0014-5793(91)80715-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 190 kDa protein was purified from conditioned media of mouse marrow-derived stromal cell (ST2) cultures treated with 1α 25-dihydroxyvitamin D₃ (1α,25(OH)₂D₃) and identified as the third component of mouse complement (C3). Northern and Western blot analysis revealed that the production of C3 by ST2 and primary osteoblastic cells was strictly dependent on 1α,25(OH)₂D₃, but the production by hepatocytes was not. Adding 1α,25(OH)₂D₃ together with mouse C3 antibody to bone marrow cultures greatly inhibited the formation of tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like multinucleated cells. Adding C3 alone induced no TRAP-positive cell formation. These results suggest that, in bone tissues, C3 is specifically produced by osteoblasts in response to 1α,25(OH)₂D₃ and somehow involved in inducing differentiation of bone marrow cells into osteoclasts in concert with other factors produced by osteoblasts in response to 1α,25(OH)₂D₃.

【 授权许可】

Unknown