A novel γ subunit of the GABAA receptor identified using the polymerase chain reaction

FEBS Letters

Siegel, Ruth E.¹  Wilson-Shaw, Donna²  Sikela, James M.²  Robinson, Misi²  Gambarana, Carla¹ 

[1] Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio, OH 44106, USA;Department of Pharmacology, University of Colorado Health Sciences Center, Denver, Colorado, CO 80262, USA

关键词: GABAA receptor;    Ligand-gated ion channel;    Polymerase chain reaction;   

DOI  :  10.1016/0014-5793(91)80687-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have utilized a polymerase chain reaction (PCR) strategy to identify a novel subunit, γ3, of the GABA_A receptor. The γ3 cDNA encodes a mature protein of 450 amino acids that contains structural features typically conserved among subunits of the GABA_A receptor family. The γ3 subunit shares approximately 66% sequence identity with the γ2 subunit but only 38% and 29% with α1 and β1 subunits, respectively. Localization of the γ3 mRNA indicates that it is widely distributed throughout the mouse brain in a pattern similar to that observed for mRNAs encoding the γ2 subunits.

【 授权许可】

Unknown