Metaphase chromosomes from mammalian cells stimulate fusion of artificial phospholipid vesicles

FEBS Letters

Belyaev, N.D.¹  Budker, V.G.¹  Kiseleva, E.V.¹  Balakireva, L.A.¹ 

[1] Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, pr. Lavrentieva 8, Novosibirsk 90, 630090, USSR

关键词: Chromosome: Liposome: Resonance energy transfer;    Fusion;    DPPC;    [14C]dipalmithoylphosphatidylcholine;    PC;    phosphatidylcholine;    NBD-DPPE;    N-4(-nitrobenz-2-oxa-1;    3-diazole-7yl)-dipalmithoylphosphatidylethanolamine;    Rhd-DPPE;    N-(rhodamine B)-dipalmithoylphosphatidylethanolamine;    TLC;    thin-layer chromatography;    Tris;    Tris(hydroxyethyl)aminomethane;    EDTA;    ethylendiaminetetraacetic acid;    RET;    resonance energy transfer;   

DOI  :  10.1016/0014-5793(91)80685-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Isolated mitotic chromosomes are able to form complexes with phosphatidylcholine liposomes in the presence and absence of Ca²⁺ ions, in the latter case in the presence of polyamines. Interactions with chromosomes stimulates liposome fusion. The fusion is promoted by condensed and EDTA-decondensed chromosomes.

【 授权许可】

Unknown