Sub‐picosecond dynamics of excited state of primary electron donor in reaction centers of Rhodopseudomonas viridis as revealed by hole burning at 1.7K broad and narrow holes

FEBS Letters

Shuvalov, V.A¹  Shkuropatov, A.Ya¹  Ganago, A.O¹ 

[1] Institute of Soil Science and Photosynthesis, USSR Academy of Sciences, Pushchino, Moscow region, 142292, USSR

关键词: Reaction center;    Primary electron donor;    Hole burning;    Rhodopseudomonas viridis;    BL and BM;    bacteriochlorophyll monomers located in L and M protein subunits;    respectively;    HL and HM;    bacteriopheophytins in L and M subunits;    respectively;    QA and QB;    primary and secondary quinones;    respectively;    Qγ transition;    long-wavelength transition of pigments;    S-factor;    Pekar-Huang-Rhys factor;    ZPH;    zero-phonon hole;   

DOI  :  10.1016/0014-5793(91)80684-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Within the Q_γ band of the primary electron donor (P), the spectra of absorbance changes due to the formation of a state P⁺Q_A ⁻ (Q_A is the primary quinone) at 1.7K in Rhodopseudomous viridis reaction centers excited at 1014 nm have been shown to involve two spectral features characterized by: (i) a progression of broad (170–190 cm⁻¹) Gaussian vibronic bands (S-factor = 1.4) separated by 150 cm⁻¹ and (ii) a ‘narrow’ structure near 1014 nm, characterized by 0-0 transition at 1014 nm with a width of ∼50 cm⁻¹ and 0–1 transition at 1000 nm with the width of ∼100 cm⁻¹, and S-factor = 0.9. The width of 50 cm⁻¹ can be related to either zero-phonon hole (ZPH) width or the structure involving phonon wings and ZPH being unresolved. Since dichroic value (∼0.37) is unvarying over the P band, the vibrations involved are totally symmetric. The ZPH (width of ∼3 cm⁻¹) and phonon wings (frequency of ∼30 cm⁻¹) are resolved within the P band near 1014 nm when the spectrum of ΔA due to the formation of bacteriopheophytin_L ⁻ is measured at 1.7K.

【 授权许可】

Unknown