Atropine dissociates complexes of muscarinic acetylcholine receptor and guanine nucleotide‐binding protein in heart membranes

FEBS Letters

Matesic, Diane F.¹  Luthin, Gary R.² 

[1]Department of Anatomy, University of Pennsylvania, Hahnemann University, Philadelphia, PA 19102, USA

[2]Department of Physiology and Institute for Neuroscience, Hahnemann University, Philadelphia, PA 19102, USA

关键词: Muscarinic acetylcholine receptor;    G protein;    Atropine;   

DOI  :  10.1016/0014-5793(91)80680-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Complexes of muscarinic acetylcholine receptor and guanine nucleotide-binding protein (G protein) are formed in the presence of the agonist carbachol. The complexes remain stable after removal of agonist, and survive subsequent solubilization from cardiac membranes and purification. Dissociation of the receptor from the G protein occurs when the antagonist atropine is added following removal of agonist. This is the first direct demonstration of destabilization of receptor—G protein complexes by the binding of an antagonist.

【 授权许可】

Unknown