Subcellular distribution of enzymes involved in the biosynthesis of cyanelle murein in the protist Cyanophora paradoxa

FEBS Letters

de Pedro, Miguel A.²  Plaimauer, Barbara¹  Löffelhardt, Wolfgang¹  Pfanzagl, Beatrix¹  Berenguer, José² 

[1] Institut für Allgemeine Biochemie der Universität Wien and Ludwig-Bolzmann-Forschungstelle für Biochemie, Währingerstrasse 38, A-1090 Vienna, Austria;Centro de Biologia Molecular C.S.I.C.-U.A.M. & Facultad de Ciencias U.A.M., Campus de Cantoblanco, E-28049 Madrid, Spain

关键词: Cyanophora paradoxa;    Cyanelle;    Peptidoglycan biosynthesis;   

DOI  :  10.1016/0014-5793(91)80677-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cyanelle containing organisms, notably Cyanophora paradoxa, best studied among them, are unique with respect to the occurrence of peptidoglycan (murein) within an eukaryotic cell. Enzyme activities involved in the biosynthesis of UDP-N-acetyl-muramylpentapeptide could be localized within the cyanelle compartment. Some of the enzymes performing later steps of murcin biosynthesis were detected in the postcyanelle supernatant rather than in the cyanelle lysate. This is taken to reflect a ‘periplasmic’ location of these enzymes that are partially liberated upon rupture of the cyanelle outer membrane.

【 授权许可】

Unknown