Extended N‐terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria

FEBS Letters

Choli, Theodora¹  Kruft, Volker¹  Graack, Hanns-Rüdiger¹  Grohmann, Lutz¹  Goldschmidt-Reisin, Sabine¹  Kitakawa, Madoka¹ 

[1] Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Ihnestr. 73, D-1000 Berlin 33, Germany

关键词: Mitoribosomal protein;    Amino acid sequencing;    Mitochondria;    Yeast;    Saccharomyces cerevisiae;    PVDF;    polyvinylidene difluoride;    TP50;    total proteins of the 50 S mitochondrial ribosomal subunit;    PAGE;    polyacrylamide gel electrophoresis;    MRP;    mitochondrial ribosomal protein gene;    SDS;    sodium dodecylsulphate;   

DOI  :  10.1016/0014-5793(91)80759-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have determined the N-termini of 26 proteins of the large ribosomal subunit from yeast mitochondria by direct amino acid micro-sequencing. The N-terminal sequences of proteins YmL33 and YmL38 showed a significant similarity to eubacterial ribosomal (r-) proteins L30 and L14, respectively. In addition, several proteins could be assigned to their corresponding yeast nuclear genes. Based on a comparison of the protein sequences deduced from the corresponding DNA regions with the N-termini of the mature proteins, the putative leader peptides responsible for mitochondrial matrix-targeting were compiled. In most leader sequences a relative abundance of aromatic amino acids, preferentially phenylalanine, was found.

【 授权许可】

Unknown