Energetic cooperation via ion‐permeable junctions in mixed animal cell cultures

FEBS Letters

Boitsova, L.Ju.¹  Kublik, L.N.³  Marachova, I.I.²  Potapova, T.V.¹  Chailakhyan, L.M.³  Vinogradova, T.A.²  Aslanidi, K.B.¹ 

[1] A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, 199899 Moscow, USSR;Institute of Cytology, Academy of Sciences USSR, 194064 Leningrad, USSR;Institute of Biological Physics, Academy of Sciences USSR, 142292 Pushchino, Moscow Region, USSR

关键词: Intercellular junction;    Intracellular ion content;    Cell culture;    Ouabain;    Energy transfer;   

DOI  :  10.1016/0014-5793(91)80612-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Low ouabain concentration (1 × 10⁻⁴ M) is shown to decrease intracellular K⁺ (K⁺ _in) and to increase intracellular Na⁺ (Na⁺ _in) in human fibroblast cell cultures. The same ouabain concentration was without effect upon K⁺ _in ad Na⁺ _in in rodent cultures such as BHK-21, mouse fibroblasts and rat glyoma C6 cells. K⁺ _in and Na⁺ _in in the mixed cultures of human and BHK-21 fibroblasts or human and mouse fibroblasts were found to be resistant to 1 × 10⁻⁴ M ouabain whereas that of the mixtures of human and rat glyoma C6 cells proved to be ouabain-sensitive. The gap-junction-mediated dye transfer was revealed between human and BHK-21 cells. Such an effect was very small in the human-C6 cell mixed culture. It is concluded that cells with active ion pumps can support the maintenance of K⁺ and Na⁺ gradients in cells with inactive pumps, provided that effective ion transport via gap junctions takes place.

【 授权许可】

Unknown