Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31

FEBS Letters

Matsunaga, Tadashi¹  Burgess, J.Grant¹  Mihashita, Hideaki¹  Miyake, Masato¹ 

[1] Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184, Japan

关键词: Marine Rhodobacter;    Plasmid;    Replication region;    Sequence;   

DOI  :  10.1016/0014-5793(91)80603-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The minimum region required for the replication of the marine Rhodabacter endogenous plasmid pRD31 has been sequenced. This region is located on a 1367-bp HincII-PstI restriction fragment and is 62% rich in G-C base pairs. A region homologous to the bacteriophage P1 repA promoter, which overlaps two inverted repeats, has been identified. Plasmids with mutations in this 1367-bp region could not replicate in marine Rhodobacter hosts. This is the first identified replication origin of a photosynthetic bacterium.

【 授权许可】

Unknown