rig encodes ribosomal protein S15 The primary structure of mammalian ribosomal protein S15

FEBS Letters

Teraoka, Hiroshi¹  Itoh, Takako¹  Kitagawa, Motoo²  Yamamoto, Hiroshi²  Kikuchi, Norihisa¹  Okamoto, Hiroshi²  Takasawa, Shin² 

[1] Shionogi Research Laboratories, Shionogi & Co. Ltd., Fukushima-ku, Osaka 553, Osaka, Japan;Department of Biochemistry, Tohoku University School of Medicine, Sendai 980, Miyagi, Japan

关键词: rig;    rig protein;    Ribosomal protein S15;    Amino acid sequence;    N-terminal modification;    Rat liver;    PVDF;    polyvinylidene difloride;    PTH;    phenylthiohydantoin;   

DOI  :  10.1016/0014-5793(91)80590-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

rig, a gene originally isolated from a rat insulinoma cDNA library, codes for a basic 145 amino acid protein [(1986) Diabetes 35, 1178–1180]. Here we show that the immunoreactivity to a monocional antibody against the deduced rig protein and the translation product of rig mRNA comigrated with ribosomal protein S15. The amino acid sequence of ribosomal protein S15 purified from rat liver coincided with that deduced from the nucleotide sequence of rig mRNA, but there were indications that the initiator methionine was removed and the succeeding alanyl residue was monoacetylated. From these results, we conclude that the product of rig is ribosomal protein S15.

【 授权许可】

Unknown