Bidirectional effects of Kupffer cells on hepatocyte proliferation in vitro

FEBS Letters

Meyer, Dieter H.¹  Bachem, Max G.¹  Gressner, Axel M.¹ 

[1] Department of Clinical Chemistry and Central Laboratory, Philipps-University, Baldingerstraße, W-3550 Marburg, Germany

关键词: Cell interactions;    Kupffer cell;    Hepatocyte;    Proliferation;    Transforming growth factor α;    β;    BSA;    bovine serum albumin;    DMEM;    Dulbecco's modification of Eagle's medium;    EGF;    epidermal growth factor;    FCS;    fetal calf serum;    KC;    Kupffer cells;    KCcm;    Kuppfer cell conditioned medium;    MFblC;    myofibroblast-like cells;    MFblCcM;    myofibroblast-like cell conditioned medium;    PC;    parenchymal cells;    PL;    perisinusoidal lipocytes;    TGF;    transforming growth factor;   

DOI  :  10.1016/0014-5793(91)80574-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The control of rat hepatocyte DNA synthesis in vitro by Kupffer cells and transformed perisinusoidal lipocytes, i.e. myofibroblast-like cells was studied. Conditioned media from Kupffer cells inhibit the replicative (hydroxyurea-sensitive) DNA synthesis dose-dependently in primary cultures of hepatocytes stimulated by epidermal growth factor (EGF). The cytokine responsible for the inhibition was identified as transforming growth factor β (TGFβ). After neutralization of activated TGFβ in these media. DNA synthesis is stimulated in quiescent hepatocytes via transforming growth factor α (TGFα) demonstrated by competitive TGFα/EGF-receptor blocking on hepatocytes. Results similar to those obtained with Kupffer cells were found with conditioned media of myofibroblast-like cells. Northern blot hybridization confirms the expression of both TGFβ and TGFα in Kupffer cells and myofibroblast-like cells, respectively. These findings support the notion that Kupffer cells and myofibroblast-like cells might regulate in both directions liver regeneration depending on the proportion of secreted TGFα and TGFβ and on the activation status of TGFβ, of which a significant fraction is secreted in the latent form.

【 授权许可】

Unknown