Cultured human keratinocytes cannot metabolize vitamin D3 to 25‐hydroxyvitamin D3

FEBS Letters

MacLaughlin, Julia A.¹  Castonguay, Maurice A.¹  Holick, Michael F.¹ 

[1] Boston University School of Medicine, Boston, MA, USA

关键词: Cultured human keratinocyte;    Vitamin D3;    25-Hydroxyvitamin D3;   

DOI  :  10.1016/0014-5793(91)80525-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The metabolism of [³H] vitamin D₃ was studied in cultured human keratinocytes (CHK). Intact CHK were incubated for 1, 6, 12, 24 and 48 h with [³H] vitamin D₃ and the lipid soluble fractions from the media and cells were extracted by high-performance liquid chromatography (HPLC). Vitamin D₃ and its metabolites, 25-OH-D₃, 24,25(OH)₂D₃ were added to the extracts, as markers, prior to HPLC, HPLC analysis of the lipid extracts did not reveal any monohydroxylated metabolites. CHK incubated for one hour with [³H]25-OH-D₃ showed a 10±4% conversion to [³H]1,25,(OH)₂D₃ whereas no conversion to [³H]1,25(OH)₂D₃ was observed in control CHKs that were boiled prior to incubation with [³H]25-OH-D₃. These findings suggest that cultured neonatal keratinocytes are incapable of metabolizing vitamin D₃ to 25-OH-D₃.

【 授权许可】

Unknown