Purification and partial characterization of glyoxalase I from a higher plant Brassica juncea

FEBS Letters

Deswal, Renu¹  Sopory, Sudhir K.¹ 

[1] Plant Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110 067, India

关键词: Glyoxalase I;    Enzyme purification;    Methylglyoxal;    Glutathione;    S-hexyl glutathione;    GSH;    reduced glutathione;    MG;    methylglyoxal;    PMSF;    phenyl methylsulfonylfluoride;    PVP;    polyvinylpyrroliodone;    EGTA;    ethylene glycol-bis(β-aminoethyl ether)N;    N;    N′;    N′-tetra acetic acid;   

DOI  :  10.1016/0014-5793(91)80494-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The glyoxalase I was purified from Brassica juncea by affinity chromatography on S-hexyl GSH sepharose 4B, Homogeneity of the protein was confirmed electrophoretically by a silver stained gel. Activity staining on a native starch gel also showed a single band. The effect of glutathione, methylglyoxal, and pH on enzyme kinetics was studied. Magnesium was found to stimulate the enzyme activity.

【 授权许可】

Unknown